Modulation of Adhesion Molecule Expression on Prostate Tumor Cells after Co-Culture with Eosinophilic Cell Lines
Abstract
We studied the effect of eosinophil 24hr. cultured supernatants and exogenous cytokines on growth and adhesion molecule expression on PC3, DU145 and LNCaP prostate cancer (Pca) cell lines. Hypo and hyperdense peripheral blood eosinophils (Eos) significantly inhibited LNCaP, PC3 and DU145 cell growth in vitro. This activity was enhanced by IL-S. DU145 and PC3 colony formation was inhibited by 50-75% by Eos cell lines, while peripheral blood Eos inhibited PC3 colony formation by 95%. 24hr. cultured supernatants significantly inhibited PC3 colony formation, causing 100% inhibition, and were more variable in their effects on DU145 (inhibition ranging from 9-100%). IL-4 and TNF-alpha inhibited PC3 and DU145 growth. ICAM-1, was upregulated on PC3 and DU145 by IL-1-alpha and TNF-alpha. IL-lO upregulated vCAM-1, ELAN, and E- Cadherin on PC3, while IL-12 upregulated ELAN and E-Cadherin. 24hr. supernatants had no measurable effect on adhesion molecule expression, however Eos:DU145 co-culture(E:T ratio 1:1) resulted in an upregulation of E-Cadherin on these cells. IDV density measurements of tumor cell cultures post Eos and conditioned supernatant treatment confirmed the inhibitory effects observed. The upregulation of E-Cadherin by Eos and IL-12 is intriguing. These observations may be useful for therapeutic strategies in prostate cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2001
- Accession Number
- ADB282110
Entities
People
- Paulette M. Furbert-harris
Organizations
- Howard University