Growth Inhibition of Breast Tumor Cells by Hypodense and Normodense Eosinophilic Cell Lines
Abstract
We hypothesized that activated eosinophils (Eos) can inhibit the growth of breast tumor cells in vitro, and that this due in part to the action of released mediators. In this study we utilized Eos cell lines, previously developed from peripheral blood of allergic/asthmatic individuals, and newly developed sublines (FACS sorting of parent lines using antibodies to Eos markers). Using both monolayer culture and colony forming assays, we have demonstrated that both hypodense (.22) and hyperdense (.24) cell lines and sublines markedly inhibited MCF-7 and MDA-MB-231 tumor cell growth. Inhibition of colony formation ranged from 3% to 100% at E:T ratios 10:1 to 1000.1. Using an integrated density value (IDV) measurement of the cell density in the monolayer cultures, inhibition ranged from 0% to 75% at E:T ratios 1:1 to 5:1 Conditioned supernatants, containing TNF-alpha and IL-4 inhibited colony formation by as much as 100%. These data clearly demonstrate the cytotoxic activity of Eos hypodense and hyperdense cell lines (comparable to activated peripheral blood eosinophils) which bind to tumor cells, release mediators that kill them. The establishment of Eos cell lines and sublines offer a rich resource for future studies on the characterization of the mediators (bot) protein and molecular characterization), the binding requirements and the overall biologic role of Eos as anti-cancer effectors.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2001
- Accession Number
- ADB282177
Entities
People
- Paulette M. Furbert-harris
Organizations
- Howard University