Role of Human DNA Polymerase and Its Accessory Proteins in Breast Cancer
Abstract
Besides using immunoafffinity chromatography techniques to show that the deregulation of the cell cycle machinery in breast cancer cells, we have been studying the mechanism of genomic instability in breast cancer cells in a multifaceted manner. A few novel interactions were detected. In this study we found that p21 not only interact with PCNA but also interact directly with DNA polymerase delta p50 subunit. The tumor suppressor Rb also interacts with pol delta. Using yeast two hybrid and proteomic approaches we also found a few novel proteins that interact with polymerase delta and Replication Factor C subunits using MCFlO A and N4CF delta breast cancer cell lines. These novel findings broaden our traditional thinking about the regulation of DNA replication, repair and recombination. These studies provide a deeper understanding of the linkage between the regulation of pol delta and its accessory proteins and carcinogenesis in breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2002
- Accession Number
- ADB282182
Entities
People
- Marietta Y. Lee
Organizations
- New York Medical College