Identification of Novel Prognostic Genetic Marker in Prostate Cancer
Abstract
Prostate cancer (PCa) has become the most commonly diagnosed cancer in men in North America. A critical issue in the management of PCa has been the lack of DNA-based markers for early detection and subsequent cure, before the disease can disseminate and become life-threatening. Our research premise is that analysis of chromosomal alterations in PCa, when correlated with clinical parameters, will provide a better understanding of the disease initiation and progression. FISH analyses of HPIN samples showed that the overall level of numeric chromosomal abnormalities, especially of chromosome 8, increased in patients that subsequently progressed to carcinoma. The presence of p53 mutation in HPIN was associated with the presence of CIN as determined by FISH. SKY analyses of PCa cell lines identified a consistent pattern of CIN underlying tumorigenesis with recurrent submicroscopic deletions occurring on chromosome 8p. In addition, CGH analyses of microdissected DOP-PCR amplified PCa foci demonstrated a more complete repertoire of aberrations as well as a better phenotype-genotype correlation. Presently, we are developing a high-resolution microarray CGH approach to focus on gene dosage changes on chromosome 8. Together, these results will aid in tumor suppressor gene(s) discovery that will eventually be used as prognostic markers of PCa progression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2001
- Accession Number
- ADB282264
Entities
People
- Jeremy Squire