The Role of Rho GTPase and Trio in erbB Receptor Mediated Cellular Response
Abstract
Uncontrolled cell proliferation is a predominant feature of cancer. In fact, it is the deregulation of signaling pathways that control cell proliferation that lead to the course of oncogenic transformation and tumorigenesis. The ErbB families of growth factor receptors (ErbB1-4) and their ligands have been strongly implicated in the genesis of a number of human carcinomas. The ErbB receptors mediate cellular responses to growth factors through their intracellar signaling domain. Therefore, it is critical to identify and characterize the effectors associated with the phosphorylated C-terminal tail of activated ErbBs and the effectors mediated the ErbB-dependent signals to the downstream mitogenic signaling pathways such as BMKI cascade. For this one- year project, we have systematically fished out effectors that interact with the signaling domains of ErbBs and molecules that bind to MEKK3, the upstream regulating kinase for BMK1 pathway by yeast two-hybrid system. In addition, we have generating recombinant proteins of some of these interacting protein and establishing binding assays for analyzing their binding to the targeted molecules in mammalian cells. These studies should provide fundamental information about the mechanism of ErbB-mediated breast cancer development and could offer new therapeutic strategies for preventing the progression of breast cancer in the clinic.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2002
- Accession Number
- ADB282720
Entities
People
- Jiing-dwan Lee
Organizations
- Scripps Research