Identification and Characterization of Internalization Signal of the Prostate Specific Membrane Antigen
Abstract
The purpose of this award was to identify and characterize an internalization signal in the cytoplasmic tail of prostate specific membrane antigen (PSMA, transmembrane protein abundantly expressed in prostate cancer cells. These studies should aid in understanding the mechanism of antibody uptake and fate of the internalized antibody to improve antibody delivery approaches for immunotherapy for prostate cancer., We have now established and standardized a culture model to study internalization signal of PSMA. In addition we have determined that the cytoplasmic tail of PSMA has an internalization signal. An alanine scan mutagenesis approach in which each of the amino acids in the cytoplasmic tail is mutated to an alanine indicated that a di-leucine like motif in the cytoplasmic tail of PSMA might be involved in its internalization. Further mutagenesis approaches revealed that the N-terminal first five amino acids forms a novel endocytic determinant that render internalization function to a non-internalized protein. Thus we have now conclusively determined the endocytic determinant of PSMA.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2001
- Accession Number
- ADB283519
Entities
People
- Ayyappan K. Rajasekaran
Organizations
- University of California, Los Angeles