Molecular Mechanisms of pRB Function in Differentiation Contributing to pRB Mediated Tumor Suppression
Abstract
Inactivation of normal function of the retinoblastoma protein (pRE) contributes to the majority of cancers including breast cancer. The broad objective of this research is to understand the impact of promotion of differentiation by pRB to its tumor suppression function. To accomplish this goal, we need to understand first function of pRB in differentiation at the molecular level. We aimed to identify proteins with which pRB interacts to promote differentiation. In screenings using Dual Bait System, we identified several cellular proteins that still interact with the pRB mutants associated with a low risk of retinoblastoma. Unlike high risk of cancer mutants, these mutants retain the ability to promote differentiation. To understand role of the identified proteins in differentiation, we started with pRE-Binding Protein 2 (RBP2) whose homolog, PLU-l, has been shown to be closely associated with the malignant phenotype in breast cancer. We propose that disregulation of RBP2 interaction with pRB in the cause of mutation may lead to cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2002
- Accession Number
- ADB283904
Entities
People
- Elizaveta Benevolenskaya
Organizations
- Dana–Farber Cancer Institute