Centrosome Hypertrophy Induced by p53 Mutations Leads to Tumor Aneuploidy
Abstract
This research is designed to test the hypothesis that aneuploidy in some breast tumors is caused by centrosome abnormalities which are induced by alteration in p53 function. Specific mutations in p53 that are associated with breast cancer, aneuploidy, chromosomal instability, and centrosome abnormalities have been identified during the course of this project. We have shown that p53 mutations correlate with an increase in microtubule nucleating capacity; however, no such correlation is indicated with either centrosome size or centrosome number. To test whether or not specific p53 mutations affect different aspects of centrosome function, thus leading to chromosomal instability and/or changes in microtubule nucleation, we have constructed 5 different adenoviral vectors for our mutant p53 studies. Initial experiments with 2 of these mutant p53 vectors indicates that specific p53 mutations do have different effects on centrosome function in cultured cells derived from normal human mammary epithelia. Further experiments are in progress or in the design stage.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2002
- Accession Number
- ADB285530
Entities
People
- Wilma L. Lingle