Tissue Specific Activation and Inactivation of the Neu Proto-Oncogene in Transgenic Mice Using Cre Recombinase
Abstract
Breast cancer is a prevalent, yet poorly understood disease. Of the women afflicted with breast cancer, 20-30 % of cases contain amplified and overexpressed ErbB2 (Neu, HER2). In order to examine the role of ErbB2 in mammary gland development and tumorigenesis, I have sought to both create mice containing a mammary specific knockout of ErbB2 and a mammary specific activation of the oncogenic form of ErbB2. To achieve these goals, a line of transgenic mice expressing Cre Recombinase in the mammary epithelium was created. These mice have been interbred with mice containing a loxP flanked erbB2 cDNA in place of exon one at the genomic erbB2 locus. This resulted in a delay in ductal outgrowth in the early stages of mammary gland development. However, by 6 weeks of development, the mammary glands lacking ErbB2 are indistinguishable from wild type glands and appear to lactate normally.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2002
- Accession Number
- ADB286185
Entities
People
- Eran R Andrechek
- William A Muller
Organizations
- McMaster University