Magnetic Separation Device.
Abstract
This invention pertains to magnetic separation devices and more particularly to; magnetic separation devices used to selectively remove magnetic bead-coated cells from tissues such as bone marrow or blood. Depletion of cell populations from boner marrow has been mainly approached using antibodies conjugated to toxins such as ricin or with antibodies complement to effect lysis of the target cells. These techniques have severals disadvantages including difficulty in measuring the selective cell kill in the marrow, non-specific toxicity of either toxins or complement, and the necessity to prepare large amounts of complement. In addition, many antibodies are neither cytotoxic with complement nor toxin conjugates. Polymeric microspheres conjugated to antibodies have been used to probe the cell surface for receptor sites using scanning electron microscopy. Iron-containing polymeric microspheres tagged with fluroescent dyes conjugated to antibodies were used to separate red blood cells and lymphoid cells by binding the antibody-microsphere to selected cells and exposing the cell population to a magnetic field. Over 99% of the bound cells were attracted by the magnet.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 25, 1985
- Accession Number
- ADD012163
Entities
People
- Charles A. Twigg
- Duncan P. Crawford
- George M. Starken
- J. Cavin
- Richard A. Quinnell
Organizations
- United States Department of the Navy