Quantitative Immunochromatographic Strip Assay Method and Apparatus.
Abstract
This patent application discloses an immunochromatographic assay for quantitation of a biological substrate such as hepatitis B antibody in which strips of nitrocellulose acetate filter paper are uniformly coated with a specific binding material for the substrate, such as hepatitis B surface antigen, after which non-specific binding sites on the strips are blocked with proteinaceous material, such as serum albumin. Sample solutions containing unknown amounts of substrate are allowed to migrate up each strip so that the specific binding material will bind the substrate, the area occupied by bound substrate corresponding to the concentration of substrate in the sample solution. The presence of bound substrate is determined by immersing in a solution containing a complex of specific antibody for the bound substrate, such as anti-immunoglobulin, and an indicator activating agent, such as peroxidase enzyme. The strips are then rinsed to remove excess unbound complex, and immersed in an indicator solution, such as a solution of 4-chloro-1-naphthol and hydrogen peroxide. Peroxidase mediated oxidation of the indicator produces a dark blue chromophore precipitate marking the presence of substrate on the strip.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 18, 1985
- Accession Number
- ADD012173
Entities
People
- Jack W. Vincent
- John E. Van Hamont
- Michael G. Pappas
- Ruta Hajkowski
Organizations
- United States Army