A Membrane Based Dot Immunoassay and Method of Use.
Abstract
Antigens or antibodies are detected using a novel membrane based immunoassay. Known antigens or antibodies which will form complexes with antigens/antibodies to be assayed are spot filtered with pressure through a membrane. The membrane, either by itself or attached to a base material as a test strip, is incubated with a test fluid. Consequently, the resulting antibody-antigen complex is incubated directly or after an intermediate anti-antibody incubation with enzyme conjugated immunoglobulin and exposed to substrate which produces a colored insoluble product if the test target is present. Patent applications. (aw)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 30, 1989
- Accession Number
- ADD014505
Entities
People
- John J. Oprandy
Organizations
- United States Department of the Navy