Pseudomonas Chlororaphis Microorganism, Polyurethane Degrading Enzyme Obtained Therefrom and Method of Using Enzyme,
Abstract
Wild-type bacteria with polyurethanase activity, and the ability to excrete polyurethanase into a culture medium have been genetically altered to significantly increase their polyurethanase activity. Biologically pure cultures of these genetically altered are cultured in a inedium containing an inducer for the production of polyurethanase. The bacteria are then removed from the culture medium to provide a preparation with a high polyurethanase activity. One useful genetically altered bacteria is a strain of Pseudomonas chloroaphis . The polyurethanase-containing preparation may then be used directly to degrade polyurethane, particularly polyurethane and polyurethane- based coatings.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 31, 1995
- Accession Number
- ADD017494
Entities
People
- James R. Campbell
- Joel R. Crabbe
- Michael T. Montgomery
- Steven E. Walz
Organizations
- United States Department of the Navy