Application of Flow Cytometric Methods in Immunotoxicology
Abstract
Flow cytometry is an instrumental technique that permits the simultaneous, quantitative measurement of a variety of properties on individual cells in a flow stream at rates of several thousand cells per minute. In addition, many instruments provide the capability to sort cells on the basis of preselected determinants. Examples of parameters that can presently be measured by commercial instruments include: single or dual wavelength fluorescence, low or 90 degrees angle light scatter, cell number, cell size or volume, and others. Fluorescence provides the basis for the majority of applications and has been utilized in the development of methods for the analysis of DNA cell cycle phase, cell surface marker expression, phagocytosis and viability in suspensions of single cells. These analytical techniques make possible the study of chemically- induced alterations in cell proliferation and differentiation, specific cell functions, such as phagocytosis and cytotoxicity, and the identification of specific cell populations.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1983
- Accession Number
- ADP001964
Entities
People
- Jack H. Dean
- Richard D. Irons
- Wayne S. Stillman