Effects of Topically-Administered Phorbol Diester Promoters on Immune Functions,
Abstract
The response of splenic leukocytes to phorbol diesters following topical administration was found to be qualitatively different from that observed in vitro. The promoters were mitogenic following topical administration, but not in cell suspensions. In vivo TPA apparently acted on a cell population other than T cells, whereas those in vitro have been suggested to be a T cell subset. The above findings suggested either that spleen cell cultures in vitro lack necessary accessory cells or factors present for TPA-induced mitogenesis, or that the effects observed in vivo are indirect responses to the promoter, possibly resulting from inflammation. This latter suggestion is also supported by the findings that splenocyte mitogenesis is also induced by mezerein, a weakly-promoting agent but irritating to a comparable degree to TPA. Mezerein, however, does show partial promoting activity in an appropriate animal model and shares many effects with TPA on cell regulation and differentiation. The finding that fluocinolone acetonide (FA) possesses both antiinflammatory and antimitogenic effects in TPA-treated animals also supports a role for inflammation in the induction of spleen mitogenesis by this agent.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1983
- Accession Number
- ADP001970
Entities
People
- C. Stuart Baxter
- R. A. Schoof
Organizations
- University of Cincinnati