Processing Enzyme Specificity is a Consequence of Pro-Hormone Precursor Protein Conformation,
Abstract
Gonadotropin associated peptide (GAP)-releasing enzyme and atrial granule serine proteinase (AGSP) which are isolated and purified from bovine hypothalamic neurosecretory granules and bovine atrial granules respectively, are likely processing enzymes of pro-gonadotropin-releasing hormone (Pro-Gn RH)/GAP and pro-atrial natriuretic factor (ANF) respectively. It was of interest to determine whether each enzyme would maintain its specificity when confronted with the processing sequence of the alternate enzyme. To this end, several peptides based on the primary sequency of pro-ANF and pro-GnRH/GAP were prepared which incorporated either the processing site sequence for the relevant enzyme for the processing site sequence for the alternate processing enzyme. Hydrolysis of these peptide substrates by both enzymes was then assessed.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1992
- Accession Number
- ADP008367
Entities
People
- N. S. Rangaraju
- R. B. Harris
Organizations
- Virginia Commonwealth University School of Medicine