Correlation of Conformation with Antibody Affinity for Fibrinogen Gamma/Chain Carboxyl Terminal Peptide Segment,
Abstract
The carboxyl terminal amino acids of the 411 residue fibrinogen gamma-chain play a significant role in blood clotting. One of the steps in the polymerization of fibrinogen to fibrin involves the intermolecular gamma-chain crosslinking of the side chains of Gln(398) and Lys(406) a process catalyzed by activated Factor XIII. The terminal portion of fibrinogen (but not fibrin) gamma-chain also stimulates platelet aggregation, this activity being contained within residues 400-411. Because of the physiological significance of the fibrinogen-gamma C-terminal region, antibodies have been prepared against it. We are using NMR to study the interaction of fibrinogen gamma-chain peptides with monoclonal antibody 4A5.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1992
- Accession Number
- ADP008372
Entities
People
- Gary R. Matsueda
- Michael Blumenstein
Organizations
- Hunter College