1H-NMR Studies of the Structure and Calcium Binding Behavior of the First EGF-Like Domain in Blood Coagulation Factor IX,

Abstract

It has been shown that there is no epidermal growth factor (EGF)-receptor binding nor mitogenic activity in the synthetic first EGF-like domain of factor IX(45-87). However, the Gla-independent calcium binding site has been located in this peptide fragment. The sequence-specific assignment of the 1H-NMR resonances and the secondary structure of the peptide have been determined by standard 2D-NMR techniques. Also, the location of the calcium binding site has been investigated.

Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1992
Accession Number
ADP008374

Entities

People

  • Arthur Pardi
  • Hong Cheng
  • James P. Tam
  • Linda H. Huang
  • William V. Sweeney

Organizations

  • Hunter College

Tags

DTIC Thesaurus Topics

  • Biological Factors
  • Blood Coagulation
  • Blood Coagulation Factors
  • Chemistry
  • Growth Factors
  • Peptides

Fields of Study

  • Chemistry

Readers

  • Aerodynamics/Aeronautics.
  • Immunology
  • Molecular and Cellular Biochemistry