The Inhibition of Fibrin Stimulated t-PA-Induced Plasminogen Activation by the A Chain Fragment 149-157 of Urokinase,
Abstract
Both urokinase (UK) and t-PA are major fibrinolytic enzymes widely used as efficient anti-thrombosis agents. The t-PA induced plasminogen activation can be strongly stimulated by fibrin. It is due to the binding both of t-PA and Glu-plasminogen to the D-domain of fibrin. The fibrin A alpha 149-161 sequence had been identified as a determinant for this stimulation. By contrast, UK (two-chain form) displayed little fibrin stimulation. A similarity of charge and hydrophobic distribution between UK 149-158 and A alpha 149-161 was found with a pattern-recognition program. The present study was undertaken to understand the structure-function relationship of UK 149-158.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1992
- Accession Number
- ADP008546
Entities
People
- A. Song
- David C. Zhu
- Jing Liu
- V. Gurewich
Organizations
- Harvard Medical School