Synthesis and Characterization of Immunoglobulin Variable Region Heavy and Light Chain Fragments,
Abstract
These syntheses of V sub L(1-63) and V sub H(1-68) of murine myeloma McPc603 represent the first automated stepwise syntheses of an antigen-binding variable region. A previous synthesis of V sub H(16-68) was performed via fragment condensation. Stepwise synthesis of the V sub L region of another antibody, MOPX 315, gave a small amount of binding activity. The F sub H(1-68) and V sub L(1-63) fragments targeted in this synthesis each contain two out of the three hypervariable loops thought to be responsible for the specificity of the antibody. Anticipated synthetic problems arose from the length of the proteins (68 and 63 amino acids) and the extreme hydrophobicity and basicity of the products. The stepwise strategy avoids problems of solubility and purification encountered in the fragment approach.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1992
- Accession Number
- ADP008557
Entities
People
- Lenore M. Martin
- R. B. Merrifield
Organizations
- The Rockefeller University