Role of Cytokines and Reactive Oxygen Intermediates in the Inflammatory Response Produced by Sulfur Mustard. A Progress Report
Abstract
Cytokines play a major role in both acute and chronic inflammatory processes, including those produced by sulfur mustard (SM). In situ hybridization of the mRNA of various cytokines with radiolabeled antisense RNA probes enables us to visualize under the microscope which cells in tissue sections of SM lesions are producing which type of cytokine. This technique, therefore, demonstrates cell function histologically, even though the cells are no longer alive at the time of analysis. Cytokines from infiltrating phagocytes. We have successfully demonstrated the mRNAs of four major cytokines in developing and healing rabbit SM lesions: Interleukin 1 beta (IL-1 beta), neutrophil attractant/activation protein 1 (NAP-1 or IL-8), monocyte chemoattractant (activating) protein 1 (MCP-1), and GRO, which is macrophage inflammatory protein 2. The macrophage/fibroblast group in the lesions contained the mRNA of all four cytokines, and granulocytes contained the mRNA of IL-1 beta and NAP-1. More cytokine producing cells were present in the peak lesions than in healing lesions.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 13, 1993
- Accession Number
- ADP008758
Entities
People
- Arthur M. Dannenberg Jr.
- Junji Tsuruta
Organizations
- Johns Hopkins University