Projected Uses of Cellular Models and Fluorescence Microscopy for Identification of Antivesicants
Abstract
Although candidate antivesicant compounds have been tested on soldiers and other volunteers during wartime, animal models have been the basis for modern studies. Recent studies indicate that human skin cells can be used as in vitro subjects for testing of antivesicants. Fluorescent dye probes and a spectrofluorometer were used to observe effects of HD (sulfur mustard) in skin models. The Cytofluor 2300 revealed evidence of cell membrane damage in skin punch samples that were collected postmortem following sulfur mustard (HD) vapor challenges to hairless guinea pigs. This study is important because it suggests that dye probe readings can be used to correlate the results from parallel in vitro and in vivo investigations. This study also supports investigation of various potential fluorescent dye applications for measurement of other in vivo biochemical lesions. Possibilities for integration of the tripartite model/dye/ Cytofluor system into the preexisting antivesicant screening and development programs have been outlined.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 13, 1993
- Accession Number
- ADP008761
Entities
People
- Laura S. Rhoads
- Millard M. Mershon
- Robert G. Van Buskirk
- Stacey M. Hachenberg
Organizations
- United States Army Medical Research Institute of Chemical Defense