Anticonvulsant Effects of Memantine and MK-801 in Guinea Pig Hippocampal Neurons.
Abstract
Memantine (1-amino-3,5-dimethyladamantane) is used in treatment of Parkinsonism and movement disorders. Recent work has demonstrated that memantine (Mem) displaces MK-801 from its binding sites, blocks NMDA receptor channels as potently as MK-801, and possesses neuroprotective properties. In this investigation we compared the anticonvulsant properties of Mem to those of MK-801 in guinea pig hippocampal slices. Extracellular recordings were obtained from area CA1 of guinea pig hippocampal slices in a total submersion chamber at 32 deg C in normal oxygenated artificial cerebrospinal fluid (ACSF). Evoked responses were elicited by stimulation of Schaffer collateral and commissural fibers. Epileptiform after discharges were induced by bath application of ACSF containing 9-16 uM NMDA or Mg2+ -free ACSF; continued application of NMDA resulted in suppression of evoked responses. Pretreatment of slices for 15-20 min with 100 uM Mem blocked epileptiform activity induced by both convulsant conditions. At this concentration Mem alone had no discernible effect on evoked responses when perfused for up to 50 min.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 13, 1993
- Accession Number
- ADP008802
Entities
People
- Frank J. Cann
- J. P. Apland
Organizations
- United States Army Medical Research Institute of Chemical Defense