A Screen for Endotoxin and Staphylococcal Enterotoxin B (SEB) Antibody in Rabbits.
Abstract
There is a well defined need for an alternate animal model to the non-human primate when studying the toxic actions of SEB. Studies of the effects of SEB using the NZW rabbit as a model are difficult to interpret because rabbits exhibit endotoxin-like symptoms when SEB is administered. In addition, they do not have an emetic response as do primates. Many earlier studies, using both monkeys and rabbits, failed to pre-screen the animals for presence of endotoxin or circulating antibodies to SEB. Prior screening allows choosing animals that are free from the interfering actions of these two variables for studying SEB effects. The approach of this study is two-fold. First is to establish parameters/methods for an ELISA to detect SEB antibodies, and determine SEB titers and endotoxin levels in naive rabbits. Secondly, we will administer SEB and look for changes in levels of these two toxins. Presently, working parameters for the ELISA to measure SEB titers have been established, and SEB titers and endotoxin levels using a microtiter assay have been measured on a small naive rabbit population.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 13, 1993
- Accession Number
- ADP008886
Entities
People
- Alan Brimfield
- Jeff Doebler
- Karen Traub
- Kim Traub
Organizations
- United States Army Medical Research Institute of Chemical Defense