A Mouse Model for Studying Toxicosis and Toxic Shock Caused by Staphylococcal Enterotoxin B.
Abstract
Monkeys are the most sensitive animal species next to humans to the intoxication caused by staphylococcal enterotoxin B (SEB). However, monkeys are costly, difficult to obtain, and variable in their responses. A mouse model of SEB intoxication was therefore developed. Mice, (BALB/c or C3H/HeJ) intraperitoneally primed with actinomycin D, became highly sensitive to intravenous SEB intoxication. The mice underwent 1-2 days of toxicosis and toxic shock and then died. Postmortem histopathology in the lung, kidney, and intestine was similar to that observed in monkeys challenged with aerosolized or intravenous SEB. Time course studies of the pathologic changes in these tissues revealed a pattern of increasingly severe inflammatory reactions. The localization of inflammation was consistent with the distribution of I-125 labeled SEB following intravenous injection. The toxicosis and histopathologic changes were closely associated with the production of monokines and lymphokines as well as the appearance of inflammatory cells in tissues. These histopathological changes might be due to direct or combined effects of SEB, lymphokines, and monokines in tissues.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 13, 1993
- Accession Number
- ADP008887
Entities
People
- Jack Komisar
- James Y. Chen
- Jeenan Tseng
Organizations
- Walter Reed Army Institute of Research