Site-Specific Attachment of gold Nanoparticles to DNA Templates

Abstract

DNA was used as a scaffold for the binding of gold nanoparticles using a standard chemical technique. A DNA template was designed with amino-modified thymines located every 3.7 nm which would allow the attachment of the carboxylic acid functionalized gold nanoparticles. The gold particles were covalently bound to the amino groups on the DNA using standard 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) chemistry in the presence of a competitor to block excess gold binding sites. The products were analyzed by transmission electron microscopy (TEM) and atomic force microscopy (AFM).

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2001
Accession Number
ADP011016

Entities

People

  • Govindarajan Muralidharan
  • Jack C. Wells
  • Jacob Barhen
  • Karen A. Stevenson
  • Leon Maya

Organizations

  • Oak Ridge National Laboratory

Tags

Communities of Interest

  • Energy and Power Technologies

DTIC Thesaurus Topics

  • Amines
  • Attachment
  • Biological Sciences
  • Carboxylic Acids
  • Chemical Reactions
  • Chemistry
  • Electron Microscopes
  • Electron Microscopy
  • Materials
  • Materials Laboratories
  • Mathematics
  • Metallic Nanoparticles
  • Methylamine
  • Microscopy
  • Nanoparticles
  • Particles
  • Quadrants

Fields of Study

  • Chemistry

Readers

  • Molecular Genetics
  • Nanoscale Plasmonic Nanotechnology

Technology Areas

  • Biotechnology
  • Microelectronics
  • Microelectronics - Graphene