Multi-Photon Fluorescence Spectroscopy of Fluorescent Bio-Probes and Bio-Molecules

Abstract

Multi-photon fluorescence spectra of a number of commonly used biological probes were measured in this study. Significant spectral variation has been detected between single and multi-photon excitation. The result is important for the proper selection of spectral setting/ dichroic beam splitter in the set-up of a multi-photon fluorescence microscope. The information can also be useful in the detection of multi-photon fluorescence in bio-chip technology. In addition, we have investigated a few highly fluorescent bio-molecules commonly found in plant cells.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2000
Accession Number
ADP011222

Entities

People

  • Bai-ling Lin
  • Chi-kuang Sun
  • Fu-jen Kao
  • Ping-chin Cheng
  • Yung-sheng Wang

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biology
  • Chemical Compounds
  • Chemistry
  • Chlorophylls
  • Detection
  • Electrical Engineering
  • Emission
  • Engineering
  • Excitation
  • Fluorescence
  • Measurement
  • Microscopes
  • Microscopy
  • Molecules
  • Optical Detection
  • Spectra
  • Spectroscopy

Fields of Study

  • Physics

Readers

  • Chemistry (specifically Chemical Fluorescence)
  • Systems Analysis and Design