Multi-Photon Micro-Spectroscopy of Biological Specimens

Abstract

The non-linear nature of multi-photon fluorescence excitation restricts the fluorescing volume to the vicinity of the focal point. As a result, the technology has the capacity for micro-spectroscopy of biological specimen at high spatial resolution. Mesophyll protoplasts of Arabidopsis thaliana and maize stem sections were used to demonstrate the feasibility of multi-photon fluorescence micro-spectroscopy at subcellular compartments. Time-lapse spectral recording provides a means for studying the response of cell organelles to high intensity illumination. KeyworeL: Micro-spectroscopy, multi-photon fluorescence spectroscopy, second harmonic generation, plant tissues, stern, chioroplast, protoplast, maize, Arabidopsis

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2000
Accession Number
ADP011224

Entities

People

  • Bai-ling Lin
  • Chi-kuang Sun
  • Fu-jen Kao
  • Ping-chin Cheng
  • Rang-wu Chen

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biospecimens
  • Cells
  • Cellular Structures
  • Detection
  • Electrical Engineering
  • Emission Spectra
  • Fluorescence
  • Intensity
  • Laser Beams
  • Lasers
  • Optical Detection
  • Protoplasts
  • Spectra
  • Spectroscopy
  • Three Dimensional

Readers

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