Multi-Photon Fluorescence Microscopy: Behavior of Biological Specimens Under High Intensity Illumination

Abstract

Recent development in multi-photon fluorescence microscopy, second and third harmonic generation microscopy (SHG and THG) and CARS open new dimensions in biological studies. Not only the technologies allow probing biological specimen both functionally and structurally with increasing spatial and temporal resolution, but also raise the interest in how biological specimens respond to high intensity illumination commonly used in these types of microscopy. We have used maize leaf protoplast as a model system to evaluate the photo-induced response of living sample under high intensity illumination. It was found that cells can be seriously damaged by high intensity NIR irradiation even the linear absorption coefficient is low in these wavelengths. Micro-spectroscopy of single chloroplast also allows us to gain insight on the possible photo-damage mechanism. In addition to fluorescence emission, second harmonic generation was observed in the maize protoplasts.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2000
Accession Number
ADP011229

Entities

People

  • Bai-ling Lin
  • Chi-kuang Sun
  • Fu-jen Kao
  • Ping-chin Cheng

Organizations

  • University at Buffalo

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biology
  • Biospecimens
  • Cell Physiology
  • Cells
  • Emission
  • Lasers
  • Light Sources
  • Materials
  • Microscopy
  • Peak Power
  • Power
  • Protoplasts
  • Sapphire
  • Second Harmonic Generation
  • Spectra
  • Spectroscopy
  • Three Dimensional

Readers

  • Chemistry (specifically Chemical Fluorescence)
  • Optical Physics and Photonics.