Thin Film Micro Arrays with Immobilized DNA for Hybridization Analysis
Abstract
In this work, a procedure to immobilize DNA probes on a microarray patterned on a flexible plastic substrate is developed. The method involves the chemical activation of a thin film surface, the introduction of amine functionality via a silanization step, the coupling of an adequate crosslinker and finally the immobilization of the DNA probe. The response of different thin-film materials and plastic substrates to the immobilization procedure is discussed. The DNA probes immobilized in the patterned pixels were then allowed to hybridize with complementary target DNA labeled with a fluorescent molecule. A prototype array of thin film pixels of SiO2 functionalized by silanization deposited over a polyimide substrate is demonstrated.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 05, 2002
- Accession Number
- ADP013611
Entities
People
- A. Faber
- D. Goncalves
- D. M. Prazeres
- F. Fixe
- R. Cabeca
Organizations
- Instituto Superior Técnico