Tissue Engineering of Fibroblast Constructs and Anisotropic Collagen Gels
Abstract
The creation of an in vitro functional tendon construct will enable testing of the influence of mechanics and nutrients on the development and remodeling of tendon under known controlled stimuli which is difficult to achieve in vivo. Tendon constructs were engineered in vitro via stress-mediated self organization of fibroblasts and ECM on a laminin coated elastomer substrate. Varying the laminin density and the amount of fetal bovine serum on the substrate affected the ability of tendon fibroblasts to form a confluent cell layer and the time to layer delamination. Understanding the factors that promote selfassembly of tendon constructs will enable their combination with already developed in vitro muscle constructs.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2002
- Accession Number
- ADP014409
Entities
People
- Ellen M. Arruda
- Karl Grosh
- Krystyna Pasyk
- Robert Dennis
- Sarah Calve
Organizations
- University of Michigan