Fiber Optic-Based Biosensor for Environmental Applications
Abstract
The NRL fiber optic biosensor is a device which measures the formation of a fluorescent complex at the surface of an optical fiber. Antibodies provide a mechanism for recognizing an analyte of interest and immobilizing a fluorescent complex onto the fiber surface. The fiber optic biosensor is fast, sensitive, and it permits analysis of hazardous materials remote from the instrumentation. Antibodies coated on the fiber are stable for up to two years of storage prior to use. A portable fiber optic biosensor has been developed which is able to monitor four fiber probes simultaneously. Quadruplicates or 4 separate samples can be analyzed with the multiple fiber system. The biosensor has been used to measure concentration of toxins, proteins, small molecules in the parts per billion (microgram/L) range in under 10 minutes. The fiber optic biosensor is currently being applied for detection of explosives, pathogens, and toxic materials which pollute the environment. On-site TNT analysis of explosive contaminated groundwater and leachate samples has been demonstrated.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1996
- Accession Number
- ADP017722
Entities
People
- Frances Ligler
- Lisa C Shriver-Lake
Organizations
- United States Naval Research Laboratory