Microfabricated 3D Scaffolds for Tissue Engineering Applications
Abstract
Microfabrication and soft lithographic techniques are combined to develop three-dimensional (3D) polydimethylsiloxane (PDMS) scaffolds comprising multiple levels of meandering pore geometry textured with 10 mum posts. Both micro-architecture and surface micro-textures have been shown to selectively stimulate cell and tissue behavior. To achieve a 3D scaffold with precise micro-architecture and surface micro-textures, 100 mum thick PDMS films were manufactured using a stacking technique to realize a 66% porous 3D structure with 200 x 400 mum horizontal through holes, 200 mum diameter vertical through holes and 71% surface coverage with 10 mum diameter and 10 mum high posts. Each PDMS porous film level was manufactured by the dual-sided molding of uncured PDMS between a three level 8U-8 photoresist mold (of 200, 10, and 100 mum thick features) and a PDMS mold with 10 mum deep micro-textures. Dual-sided molding was achieved using a custom motion control mechanical jig that allowed relative mold alignment to with ^ +/- 10 mum.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2005
- Accession Number
- ADP019707
Entities
People
- Aaron J. Fleischman
- Alvaro Mata
- Shuvo Roy