Biomedical Applications of Gold Nanoparticles Functionalized Using Hetero-Bifunctional Poly(ethylene glycol) Spacer

Abstract

To increase the targeting potential, circulation time, and the flexibility of surface-attached biomedically-relevant ligands on gold nanoparticles, hetero-bifunctional poly(ethylene glycol) (PEG, MW 1,500) was synthesized having a thiol group on one terminus and reactive functional group on the other. Coumarin, a model fluorescent dye, was conjugated to the PEG spacer and gold nanoparticles were modified with coumarin-PEG-thiol. Surface attachment of coumarin through the PEG spacer decreases the fluorescence quenching effect of gold nanoparticles. The results of cellular cytotoxicity and fluorescence confocal analyses showed that the PEG spacer modified nanoparticles were essentially non-toxic and could be efficiently internalized in the cells within one hour of incubation.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2005
Accession Number
ADP019724

Entities

People

  • Charles Dimarzio
  • Curtis Crasto
  • Dinesh Shenoy
  • Graham Jones
  • Jane Li
  • Mansoor Amiji
  • Srinivas Sridhar
  • Wei Fu

Organizations

  • Northeastern University

Tags

DTIC Thesaurus Topics

  • Alkenes
  • Breast Cancer
  • Chemical Synthesis
  • Chemistry
  • Chlorides
  • Confocal Microscopy
  • Ethylene Glycol
  • Materials
  • Materials Science
  • Metallic Nanoparticles
  • Microscopes
  • Microscopy
  • Nanoparticles
  • Nanotechnology
  • Neoplasms
  • Organic Chemistry
  • Particle Size

Fields of Study

  • Chemistry

Readers

  • Chemistry (specifically Chemical Fluorescence)
  • Molecular and Cellular Biochemistry
  • Nanocomposite Materials Science

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech