Microfluidic Cell Volume Biosensor for High Throughput Drug Screening
Abstract
The maintenance of cell volume is critical to health. Cell volume change reflects many biological and physicological processes. We have developed a lab-chip to measure cell volume change in real-time with high sensitivity and resolution, and applicable to both adherent and suspended cell populations. The volume change was detected by measuring the impedance of extra-cellular solution within a microfluidic chamber containing the cells. Using microfabrication to make precise chamber dimensions, volume change can be detected in response to an osmotic gradient <1mOsm. The sensor provides rapid screening of pharmaceutical agents affecting cell volume. We have screened for peptides that affect cell volume regulation and found one in spider venom that inhibits at ^100pM.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2005
- Accession Number
- ADP019730
Entities
People
- Daniel A. Ateya
- Frederick Sachs
- Susan Z. Hua
Organizations
- University at Buffalo