Patterning of Cells on Bioresist for Tissue Engineering Applications

Abstract

Engineering functional tissues and organs successfully depends on the ability to control cell orientation and distribution. Materials used for such purposes therefore have to be designed to facilitate cell distribution and eventually guide tissue regeneration in 3D. The field of tissue engineering hinges on developing degradable polymeric scaffolds that promote cell proliferation and expression of desired physiological behaviors through careful control of the polymer surface. The development of materials for tissue engineering and guided tissue regeneration has accelerated over the last decade.1 It has been demonstrated that non-patterned cells are effectively not tissue. "Tissues require that cells be placed and hold precise places often with precise orientations" 2-3. Cell patterning is therefore very important for tissue engineering. We have developed a biocompatible, biostable chemically amplified bioresist, with which patterns are generated without involving harsh chemical treatment. Combinatorial approach of polymer synthesis can be used to increase the number of available polymeric materials for any application and also to study the correlation s between polymer structures, material properties, and function 4. In this paper, we present a combinatorial approach for the synthesis, characterization and cell patterning using the copolymers, 3-(tert-Butoxycarbonyl)-N-Vinyl-2-Pyrrolidone-co-Methyl Methacrylate poly(MMA-co- t-BOC-NVP) and tert-butyl methacrylate-co- N-vinyl-2-pyrrolidone poly(t-BMA-co-NVP) in different compositions using free radical polymerization. Due to its hydrophilic and good biocompatibility character, N-Vinyl-2-pyrrolidone was used in the above polymer systems.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2005
Accession Number
ADP019737

Entities

People

  • Craig Halberstadt
  • K. E. Gonsalves
  • Muthiah Thiyagarajan
  • Yusif Umar

Organizations

  • University of North Carolina at Charlotte

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Alkenes
  • Carboxylic Acids
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Column Chromatography
  • Engineering
  • Ethers
  • Glass Transition Temperature
  • Hydrophilic Properties
  • Materials
  • Materials Science
  • Organic Chemistry
  • Orientation (Direction)
  • Three Dimensional
  • Tissue Engineering
  • Transition Temperature

Readers

  • Molecular and Cellular Biology
  • Polymer Science and Technology
  • Systems Analysis and Design

Technology Areas

  • Microelectronics