Labeling Cells with Silver/Dendrimer Nanocomposites
Abstract
We have developed water-soluble, biocompatible, fluorescent, and photostable silver/dendrimer nanocomposites that have a potential to be used for in vitro cell labeling. A PAMAM_E5.NH2 dendrimer was used as a template to prepare first a silver-dendrimer complex in an aqueous solution at biologic pH=7.4. Conversion into nanocomposite was achieved by irradiating the solution of the (Ag+)25-PAMAM_E5.NH2 complex by UV light to reduce the bound Ag+ to zero-valent Ag0 atoms, which were simultaneously trapped in the dendrimer network. Results indicate that the ?(Ag0)25-PAMAM_E5.NH2! silver/dendrimer nanocomposite forms positively charged single particles of 4-5 nm under the experimental conditions used. The dendrimer nanocomposite proved to be fluorescent. Toxicity testing of ?(Ag0)25-PAMAM_E5.NH2! nanocomposite revealed a behavior similar to the template dendrimer. Intracellular internalization of the silver nanocomposite and cell labeling capabilities was confirmed by confocal microscopy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2005
- Accession Number
- ADP019741
Entities
People
- Anna Bielinska
- James R. Baker Jr.
- Kai Sun
- Katarzyna Janczak
- Lajos P. Balogh
- Wojciech Lesniak
- Xiangyang Shi
Organizations
- University of Michigan