Photo-Oxidation From Mode-Locked Laser Exposure to hTERT-RPE1 Cells
Abstract
Human retinal pigment epithelial (RPE) cells (hTERT-RPE1) were used to detect photo-oxidation products generated from chronic NIR (810 nm) laser exposure. Exposure of a discrete area within cell monolayers provided a means of distinguishing fluorescence above background levels. Oxidative stress was detected using the fluorescent dye H2DCF-DA and its analog CM-H2DCF-DA. Fluorescence was detected in cells exposed to mode-locked (76 MHz, -160 femtoseconds) but not CW laser exposure. Detection of photo- oxidation from the mode-locked laser was dependent upon radiant exposure, but only if irradiance was greater than a threshold value. The CM-H2DCF-DA dye proved a more sensitive indicator of oxidation than H2DCF-DA, and the radiant exposure threshold for detection was dependent upon dye concentration. No oxidation was detected from CW exposures (using the most sensitive fluorescent dye conditions) when using 3 times the irradiance, and 10 times the radiant exposure needed to detect fluorescence from mode- locked exposure.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2004
- Accession Number
- ADP020036
Entities
People
- Benjamin A. Rockwell
- David J. Stolarski
- Debbie M. Eikum
- Gary D. Noojin
- Michael L. Denton
- Randolph D. Glickman
Organizations
- Northrop Grumman