Synthetic Remediation Biology: Chromium Upcycling for Environmental Sustainability
Abstract
Hexavalent chromium (Chromium VI, chromate) refers to compounds that contain the element chromium in the +6 oxidation state. Exposure to chromium VI compounds thru inhalation, ingestion, or direct contact is carcinogenic. Given their wide industrial usage, it is of great interest to reduce industrial wastes that contain chromium VI. Several bacteria are known to produce enzymes with chromate reductase activity. These enzymes may reduce chromium VI to chromium III. Chromium III compounds are less toxic due to its limited bioavailability. We would develop a recombinantly-expressed chromate reductase that will reduce chromium (VI) to chromium (III). Several oxidoreductases have been reported to catalyse Cr(VI) reduction in bacteria, include nitroreductase, iron reductase, quinone reductases, hydrogenases, flavin reductases as well as NAD(P)H-dependent reductases. We would use the Enzyme Function Initiative – Enzyme Similarity Tool (EFI-EST) to explore the various types of chromate reductases to identify the best candidate for industrial bioremediation. Candidates that show good chromium (VI) reductase activity will be used as the template for further enzyme evolution, using available protocols and workflows present within the NUS BioFoundry.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Aug 28, 2018
- Source ID
- FA23861814054
Entities
People
- Wen Shan Yew
Organizations
- Air Force Office of Scientific Research
- National University of Singapore
- United States Air Force