Do mechanically activated ion channels enable cellular sensing of microgravity?
Abstract
Human physiology is adapted to function under the influence of a persistent gravitational vector and extended time outside of Earth’s gravitational field can lead to adverse pathophysiological states. The molecular mechanisms by which cellular function is disrupted in microgravity are not well understood, particularly which molecular force sensors signal changes in gravity-dependent mechanical loading. Here it is proposed to combine the use of 2D and 3D tumor cell culture with the application of a microgravity state to investigate whether mechanically activated ion channels signal changes in mechanical loading in response to simulated microgravity, using a random positioning machine. This project will utilize established biological tools and experimental protocols combining imaging of cell invasion, morphology and molecular distribution and an analysis of changes in gene expression. These data will allow us to determine if mechanically activated ion channels enable cancer cells to sense changes in mechanical loading when the gravitational vector is disrupted, providing a first insight into the molecular mechanism by which cells sense gravitational changes.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jan 21, 2022
- Source ID
- FA23862114035XX0
Entities
People
- Kate Poole
Organizations
- Air Force Office of Scientific Research
- United States Air Force
- University of New South Wales