Optical Magnetic Imaging of Neuronal Currents and Impedance Using Quantum Defects in Diamond
Abstract
Networks of neurons provide the physical basis of central and peripheral nervous systems. The function of a neuronal network is determined by the properties of the constituent neurons, the spatial connectivity among them, and adaptive strengthening and weakening of those connections. Despite recent advances, however, there is not yet a clear understanding of how microscopic connectivity encodes macroscopic function, nor of the roles played by individual cells, due to the lack of tools for detailed, noninvasive interrogation of individual neurons and complex, functional neuronal networks. In the present project, the proposal aims to develop and apply a ‘quantum diamond microscope’ (QDM) for noninvasive, real-time imaging of neuronal network electromagnetic dynamics with single neuron resolution, as well as mapping neuronal currents and electrical impedances with sub-cellular resolution — capabilities that are not available with any other technology. The QDM employs nitrogen-vacancy (NV) color centers in diamond, which are atomic-scale quantum defects that enable nanoscale magnetic field sensing and imaging via optically detected magnetic resonance (ODMR). The three-year goals of the present project are (i) to optimize the QDM as a unique tool for noninvasive magnetic imaging of functional neuronal networks and single neuron bioelectric currents and impedances; and (ii) to begin application of the QDM to neurobiological problems of interest in such systems, with circuit-scale field-of-view and single cell resolution. The PI will pursue a systematic program of magnetic field sensitivity enhancement, including improvements in the NV-diamond material and the development and utilization of advanced quantum metrology techniques. The proposer also plans to incorporate NV fluorescence imaging techniques similar to those successfully used in other biological applications, and integrate the QDM with conventional optical imaging and fluorescence microscopy.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Sep 11, 2017
- Source ID
- FA95501710371
Entities
People
- Ronald L. Walsworth
Organizations
- Air Force Office of Scientific Research
- President and Fellows of Harvard College
- United States Air Force