Multidimensional Spectroscopic Probes of Heme Protein Functionality at Molecular and Cellular Scales
Abstract
Heme is an essential cofactor for almost every organism in all kingdoms of life. Hemeproteins perform a wide range of important biological functions including oxidativemetabolism, sensing of diatomic gases, cellular differentiation, gene regulation, proteintranslation and targeting, and microRNA processing. Heme proteins have also played aunique role in driving our understanding of the structure-function relationship in proteins.Heme proteins remain important model systems to advance our understanding of hownature enables the same cofactor to play vastly different functional roles by carefultuning and coupling of the electronic and vibrational degrees of freedom of the heme andits protein environment. The molecules and mechanisms of heme synthesis, transport,trafficking and signaling remain poorly understood, largely due to the lack of in vivoimaging probes for heme9. This proposal aims to advance our understanding of hemeproteins and their diverse biological roles on length scales spanning the molecular to thecellular level. At the molecular level, the proposal aims to uncover design principles usedby nature to achieve heme protein functionality, using newly developed multidimensionalspectroscopy tools to probe the protein environment and its interactions with the hemecofactor.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jun 11, 2018
- Source ID
- FA95501810343
Entities
People
- Jennifer P Ogilvie
Organizations
- Air Force Office of Scientific Research
- United States Air Force
- University of Michigan