Particle Tracking for analysis of Exosomes and other subcellular compartments for Research in TBI, PD and AD
Abstract
Our understanding of neurodegenerative diseases like traumatic brain injury (TBI),Parkinson’s or Alzheimer’s disease (PD and AD) is reliant on the ability to characterize how thedisease develops and ability to diagnose disease, especially early detection using biomarkers ofpathology as early therapeutic intervention has been shown to have the greatest promise forsuccessful chronic treatment of diseases like AD. In addition, as we continue to characterize TBI,identifying useful biomarkers that are related in initial concussive impact and damage, as well aschronic development, will have a positive effect in understanding, diagnosing and treating TBI. Theoverlap in TBI with age-related disorders like AD and PD provides added benefit to this application.This proposal requests funding to purchase the Zetaview Nanoparticle Tracking Analysis (NTA)unit that will be used to identify exosomes from various biological materials including cells forexperiments, brain tissue, CSF and blood. Exosomes are small compartments that are released from cells upon stress and contain the toxic debris of cells. We theorize that in neurodegenerative diseases like PD and AD, and recently identified in TBI, brain cells called neurons lose their ability to remove the garbage buildup in cell organelles (compartments) called autophagic vacuole, lysosome and endosome and as a result can die from when they are clogged up.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Aug 28, 2018
- Source ID
- FA95501810441
Entities
People
- Wai Haung Yu
Organizations
- Air Force Office of Scientific Research
- Trustees of Columbia University in the City of New York
- United States Air Force