Development of 3D cell culture systems for evaluating real time stimulation and sensing techniques
Abstract
Traditional cell culture consists either of two dimensional single cell type cultures grown on tissue culture polystyrene or glass, or primary tissue slices extracted from a patient and kept alive in cell culture media. For physical scientists developing bioimaging and bio stimulation techniques, these systems often lack the complexity required for validation, while the more complex tissue samples are difficult to obtain and often have short lifespans leading to irreproducibility. At the same time the 3D cell culture community needs new imaging and sensing technology that will support the real time analysis and enable biological processes and responses to be monitored without destroying the cultures. Through the development of reproducible, validated and scalable 3D cell culture models this project aims to address the common challenge facing researchers: the need for standardized and reliable model systems which can provide reproducible responses to a range of external stimuli or analysis techniques. This project aims to harness the expertise and capabilities of the collaborative team to develop, evaluate and validate 3D cell culture systems that will both provide the biophysics community with standardized and reproducible models and simultaneously explore the application of photothermal imaging and distributed nanoparticle based chemical sensing as tools for real time monitoring of 3D cell cultures.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jan 14, 2022
- Source ID
- FA95501910323
Entities
People
- Sally L. McArthur
Organizations
- Air Force Office of Scientific Research
- Swinburne University of Technology
- United States Air Force