Impedance Spectroscopy of Neuron Excitability under Electromagnetic Stimulation
Abstract
The main objective of this project is to understand the mechanisms of neuromodulation, which are critical to safe and effective performance enhancement of a healthy individual. The proposed research will be divided into three concurrent tasks for the three base years, and an additional task to take place in close collaboration with AFRL in option years 4 and 5. 1) To determine effects of stimulation on neuron impedance and excitability, and determine molecular biomarkers that will enable translatability to in vivo and human studies (including those by AFRL). 2) To create extracellular confinement that localized neural components to the detectors, and to mimic packing and conductivity of brain tissue. 3) To construct a high density detector array capable of subcellular mapping of membrane and cytoplasm impedance. 4) Array and biomarkers developed in tasks 1 3 will be used to examine effects of stimulation on human brain organoids (threedimensional constructs made of induced pluripotent stem cell derived neurons). Experiments in Task 1 will be initiated based on impedance spectroscopy and micro confinement devices that have already been established in the team’s laboratories. The experiments will then advance to examination of impedance changes at the synapses, mapping of membrane impedance in the dendrites, and shaping of the spatial distribution of extracellular potential as progress on Tasks 2 and 3 results in increased spatial resolution of the detectors. Changes in membrane impedance maps will be correlated to changes in biomarkers such as subcellular localization of ion channels and receptors, as well as morphological changes. Effects of stimulation on biomarkers and impedance changes at macro level will then be examined in human brain organoids in Task 4 in close collaboration with AFRL, especially for correlation with in vivo experiments.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jan 14, 2022
- Source ID
- FA95501910419
Entities
People
- Yevgeny Berdichevsky
Organizations
- Air Force Office of Scientific Research
- Lehigh University
- United States Air Force