A 2-PHOTON IMAGING FACILITY FOR RESEARCH AND RESEARCH EDUCATION 2019
Abstract
A Two-Photon Imaging Facility for Structural and Functional Imaging of Biological Sys- tems. Abstract: A central focus of neuroscience research is to understand how sensory and motor neu- ral networks interact as animals navigate their natural environment. The goal of our research is to characterize the role motor control systems play in modulating sensory function as animals move naturally through that environment. Understanding how such complex circuit interactions take place requires detailed characterization, of circuit architecture and more importantly of circuit function as it relates to behavioral performance. Two-photon imaging will dramatically enhance the ability of the PIs to study complex motor-to-sensory circuits and expand their AFOSR- funded research program. The aim of this proposal is to build a multi-user 2-photon imaging fa- cility for high resolution structural and functional imaging of biological tissues. The completed facility will have four Scientifica VivoScopes made for 2-photon imaging, powered by two Spec- tra Physics tunable Ti:Saphire lasers, also specifically made for 2-photon. The scopes and lasers will be mounted on two TMC 4’x8’ vibration isolations tables. West Virginia University has al- ready invested in the first table, microscope, laser and components to build the first station. This facility will be used to quantify neural network structure and function; in many cases this can be done while synchronously video recording behaviors. Data collected from this facility will accel- erate the progress of existing anatomical analysis of potential synaptic patterners and open completely new avenues and approaches to characterizing the exciting, but as yet unexplored multimodal complexities of this motor-to-sensory circuit.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Aug 12, 2021
- Source ID
- FA95502010098
Entities
People
- Kevin C. Daly
Organizations
- Air Force Office of Scientific Research
- United States Air Force
- West Virginia University