(DURIP-20) PROBING REAL-TIME SPIN DYNAMICS IN REACTIVE OXYGEN SPECIES BIOLOGY BY PULSE RESONANCE AND OPTICAL DETECTION
Abstract
This DURIP instrumentation aims to elucidate biophysical mechanisms of ROS partitioning in redox biology and the impact on cellular bioenergetics in human neural cells. We aim to study a novel domain of quantum biology: the control of the biological production of reactive oxygen species (ROS) by influencing coherent spin dynamics in a radical pair (RP) reaction. To advance fundamental understanding of ROS cellular signaling and quantum mechanisms in redox cell biology, this project seeks to augment an existing home-built hyperspectral imaging (HSI) microscope for optical detection magnetic resonance microscopy (ODMRM). The proposed ODMRM instrument adds the necessary additional capability of magnetic resonance to drive spin dynamics in RP coherences and monitor ROS products and bioenergetics by HSI. The key instrument upgrades feature innovative continuous and pulsed magnetic resonance cell stimulation, microfluidic cell cultures, multiple channel laser excitation, and Bayesian deep learning algorithms for real-time optical imaging and analysis of live cell cultures. Experiments aim to use rigorously controlled measurements and automatic feedback, deep learning algorithms to establish the mechanism of spin-dependent processes in ROS production and cellular signaling, by improving methods we have used successfully. These methodologies will advance experimental biophysics and significantly expand biophysical knowledge of quantum coherence in biology. The DURIP instrument will support education and research in experimental quantum signatures, theoretical description of quantum optimization, deep learning in microscopy, and computational modeling of quantum systems. The ODMRM technology will enable the goal of enhancing education and training for globally-engaged students with skills and knowledge that can be applied to quantum research and technologies.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Aug 12, 2021
- Source ID
- FA95502010153
Entities
People
- Robert Usselman
Organizations
- Air Force Office of Scientific Research
- Florida Institute of Technology
- United States Air Force