High-Throughput Luminometry to Support AFRL Collaborative Projects on the Interplay between Circadian Rhythms and Cellular Responses to Operational Stress
Abstract
This proposal is to acquire Actimetrics LumiCycle 32 Color and LumiCycle 96 high throughput luminometers, which do not currently exist at Wright State University (WSU). The purpose of this luminometry equipment is to provide support for current and future Air Force Research Laboratory (AFRL) collaborative projects established at WSU through CRADA and Air Force Office of Scientific Research (AFOSR) funded research activities conducted by scientists at the 711th Human Performance Wing (711HPW). The Actimetrics LumiCycles will accelerate the WSU lab’s capabilities in bringing further state-of-the-art science with respect to monitoring circadian rhythmicity in cultured cells, which will ultimately allow for ways to both better understand and ultimately improve Airmen health and performance. This system performs high-throughput luminometry on self-luminous cells and tissues from cells or animal tissues containing a transgenic luciferase reporter construct under control of a Per2 or another circadian gene promoter. Upon synchronization of the cell culture, luminescence can be continually recorded and have been shown to cycle for up to 8 days. In the short-term, the effects of Air Force-relevant external stressors on the amplitude of luciferase expression and the period length can then also be studied. In the long-term, the acquisition of this instrument will have multiple benefits- 1) Determine how external stressors impact circadian rhythms in different cell types; 2) Strengthen the growing synergy between WSU and AFRL for future joint research opportunities; and 3) Provide an excellent research-related training environment for graduate students who will provide for a talented Air Force workforce in the future.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Feb 29, 2024
- Source ID
- FA95502310080
Entities
People
- Mike Kemp
Organizations
- Air Force Office of Scientific Research
- United States Air Force
- Wright State University