Neuronal energy metabolism and synaptic function probed using subcellular, optically controlled photoactivation and photo-tagging
Abstract
This proposal seeks acquisition of essential instrumentation augmenting the capabilities of an existing microscope system to support ongoing research on low-energy bioinspired information processing. In the nervous systems, memory and other forms of information are stored as changes in the strength of synaptic connections, a form of neural plasticity. The central hypothesis is that such neural plasticity is tightly coupled to efficient energy metabolism, and seek to rigorously test this idea and determine key cellular mechanisms. The experiments use live imaging of neurons in a manner that permits the direct imaging of the cellular fuel molecule ATP along with measures of synaptic strength. The PI propose to extend the current understanding by acquiring a photostimulation system that will allow us to exploit newly developed optical probes to interrogate and control specific target molecules in a spatially and temporally precise manner. These experiments will provide essential quantitative data to improve computational models guiding the design of efficient information processing systems.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Mar 07, 2024
- Source ID
- FA95502310627
Entities
People
- Barbara Calabrese
Organizations
- Air Force Office of Scientific Research
- United States Air Force
- University of California, San Diego