Instant Evolution- Testing the Endosymbiont Hypothesis using MitoPunch Technology
Abstract
In this project, we will investigate the origin of cellular energy transduction leading to photosynthesis and respiration. We will experimentally enact conditions forcing primary and secondary endosymbiosis using MitoPunch technology, developed for cellular insertion of mitochondria, starting from the modern host and guest cells genetically proximal to putative ancestral cells. This strategy will elude the first line of celldefense mechanisms and will provide insight about early steps of endosymbiont assimilation, as well as genetic and biochemical integration. Valuable data will transpire regardless of whether or not a persistent mutualistic relationship will ensue. A separate strategy for the recapitulation of the early events of mitochondrial endosymbiosis is envisioned, in which an proteobacterial endosymbiont will be coupled to a methanogenic archaeal host cell through polyethylene glycol (PEG)-induced cell fusion. On the other hand, we will trigger selected host cells through the insertion of bioconjugates and galvanic biohybrid nanosystems based on graphene oxide. The horizontal introduction of these pseudo-organelles containing respiratory-photosynthetic-oxidative enzyme cascades will establish conditions of interim metabolic enhancement. We will also explore the effect of light stimuli, electric, magnetic and electromagnetic fields in assisting and perpetuating the viability of resulting symbionts. The proposed four-year project is a multi-disciplinary effort, bringing together experts in Nanoscience, Molecular Biology and Ecology, Materials Science and Engineering from Medgar Evers College of the City University of New York (CUNY), the University of California at Los Angeles, the University of Utah, and Rutgers, the State University of New Jersey. In this project, students at the undergraduate and graduate level, including students underrepresented in the STEM disciplines, will be introduced to cutting-edge science. A prolific intellectual exchange will ensue among the participating institutions. The research proposed herein is very timely and significant as it will provide a paradigm for future discoveries in physiological and biochemical performance enhancement.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Mar 14, 2024
- Source ID
- FA95502310737
Entities
People
- Michele Vittadello
Organizations
- Air Force Office of Scientific Research
- Research Foundation of The City University of New York
- United States Air Force